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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

CRP Polyclonal Antibody

BS-0155R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCRP
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Overview

  • Supplier
    Bioss
  • Product Name
    CRP Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1401
  • Target name
    CRP
  • Target description
    C-reactive protein
  • Target synonyms
    PTX1, C-reactive protein, C-reactive protein, pentraxin-related, pentraxin 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02741
  • Protein Name
    C-reactive protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Jin Z, Li J, Pi J, et al. Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway. Biomed Pharmacother. 2020,125:110015. doi: 10.1016/j.biopha.2020.110015
    Read this paper
  • Zhang LY , Ding L , Shi HH , et al. Eicosapentaenoic acid in the form of phospholipids exerts superior anti-atherosclerosis effects to its triglyceride form in ApoE(-/-) mice. Food Funct. 2019,10(7):4177-4188. doi: 10.1039/c9fo00868c
    Read this paper

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Figure 1. Western blot analysis of CRP using anti-CRP antibody (A00249-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CRP protein 10 ng, Lane 2: recombinant human CRP protein 5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRP antigen affinity purified polyclonal antibody (Catalog # A00249-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRP at approximately 25 kDa.
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