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Immunohistochemical staining of human liver shows strong cytoplasmic, membranous and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic, membranous and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic, membranous and nuclear positivity in hepatocytes.

Anti-CRTC3 Antibody

HPA043735
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCRTC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CRTC3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64784
  • Target name
    CRTC3
  • Target description
    CREB regulated transcription coactivator 3
  • Target synonyms
    CREB-regulated transcription coactivator 3; TORC3; TORC-3; transducer of regulated cAMP response element-binding protein (CREB) 3; transducer of regulated CREB protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UUV7
  • Protein Name
    CREB-regulated transcription coactivator 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA747623LA01HU diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of CRTC3 using anti-CRTC3 antibody (A04568-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRTC3 antigen affinity purified polyclonal antibody (Catalog # A04568-21) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRTC3 at approximately 78 kDa. The expected band size for CRTC3 is at 67 kDa.
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