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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Anti-CRYAA Antibody

HPA037737
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCRYAA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CRYAA
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1409
  • Target name
    CRYAA
  • Target description
    crystallin alpha A
  • Target synonyms
    CRYA1, CTRCT9, HSPB4, alpha-crystallin A chain, crystallin, alpha-1, heat shock protein beta-4, heat shock protein family B member 4, human alphaA-crystallin (CRYA1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02489
  • Protein Name
    Alpha-crystallin A chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CRYAA using anti-CRYAA antibody (PB9940). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse eye ball tissue lysates, Lane 3: SMMC-7721 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRYAA antigen affinity purified polyclonal antibody (Catalog # PB9940) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRYAA at approximately 20KD. The expected band size for CRYAA is at 20KD.
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