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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Anti-CTGF Antibody

AMAB91366
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CTGF Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL5339
  • Conjugate
    Unconjugated
  • Gene ID1490
  • Target name
    CCN2
  • Target description
    cellular communication network factor 2
  • Target synonyms
    CTGF, HCS24, IBP-8, IGFBP8, KMD, NOV2, SEMDLSL, CCN family member 2, IGF-binding protein 8, connective tissue growth factor, hypertrophic chondrocyte-specific protein 24, insulin-like growth factor-binding protein 8
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29279
  • Protein Name
    CCN family member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CTGF using anti-CTGF antibody (PB9541). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Thymus Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTGF antigen affinity purified polyclonal antibody (Catalog # PB9541) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTGF at approximately 38 kDa. The expected band size for CTGF is at 38 kDa.
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