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Formalin-fixed and paraffin embedded human laryngeal cancer labeled Anti-CTGF Polyclonal Antibody, Unconjugated (bs-0743R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human laryngeal cancer labeled Anti-CTGF Polyclonal Antibody, Unconjugated (bs-0743R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human laryngeal cancer labeled Anti-CTGF Polyclonal Antibody, Unconjugated (bs-0743R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

CTGF Polyclonal Antibody

BS-0743R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCCN2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CTGF Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1490
  • Target name
    CCN2
  • Target description
    cellular communication network factor 2
  • Target synonyms
    CCN family member 2; connective tissue growth factor; CTGF; HCS24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; insulin-like growth factor-binding protein 8; NOV2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29279
  • Protein Name
    Connective tissue growth factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. Chi Y et al., 2018 May, Mol Med Rep
    Read more
  • MicroRNA-145 Inhibits Cell Migration and Invasion and Regulates Epithelial-Mesenchymal Transition (EMT) by Targeting Connective Tissue Growth Factor (CTGF) in Esophageal Squamous Cell Carcinoma. Han Q et al., 2016 Oct 23, Med Sci Monit
    Read more
  • Apelin-13 protects against myocardial infarction-induced myocardial fibrosis. Zhang X et al., 2016 Jun, Mol Med Rep
    Read more
  • Biological roles of KGF, CTGF and TGF-beta in cyclosporine-A- and phenytoin- induced gingival overgrowth: A comparative experimental animal study. Al-Hamilly NS et al., 2016 Jun, Arch Oral Biol
    Read more
  • YAP activation protects urothelial cell carcinoma from treatment-induced DNA damage. Ciamporcero E et al., 2016 Mar 24, Oncogene
    Read more
  • High-salt intake suppressed microRNA-133a expression in Dahl SS rat myocardium. Guo TS et al., 2014 Jun 16, Int J Mol Sci
    Read more

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Figure 1. Western blot analysis of CTGF using anti-CTGF antibody (PB9541). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Thymus Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTGF antigen affinity purified polyclonal antibody (Catalog # PB9541) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTGF at approximately 38 kDa. The expected band size for CTGF is at 38 kDa.
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