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Anti-CTLA4 Neutralizing Antibody

Anti-CTLA4 Neutralizing Antibody

71212
BPS Bioscience
ApplicationsNeutralisation/Blocking
Product group Antibodies
TargetCTLA4
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    Anti-CTLA4 Neutralizing Antibody
  • Delivery Days Customer
    7
  • Applications
    Neutralisation/Blocking
  • Applications Supplier
    Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1493
  • Target name
    CTLA4
  • Target description
    cytotoxic T-lymphocyte associated protein 4
  • Target synonyms
    ALPS5; CD; CD152; celiac disease 3; CELIAC3; CTLA-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; IDDM12; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
  • Isotype
    IgG1
  • Protein IDP16410
  • Protein Name
    Cytotoxic T-lymphocyte protein 4
  • Scientific Description
    Neutralizing recombinant human monoclonal antibody recognizing the extracellular domain of CTLA4 and blocking binding of CTLA4 to B7.1 and B7.2. This antibody recognizes human CTLA4 but does not bind to murine CTLA4. This antibody has not been tested for cross-reactivity with other species.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-2). CTLA4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-CTLA4 Antibody (A00020-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Binding of anti-CD152/APC to recombinant CD152-coated CML beads. Methods: Anti-CD152/APC was tested for binding to CD152-muIg-coated 4micro CML beads in FACS (Inquire). Five x 10^5 CD152-muIg coated beads per tube were spun down and incubated 45 min at 4°C with 80microl of anti-CD152/APC at a 1:50 dilution (10microg/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 1.92 log10 fluorescent units when compared to a Mouse IgG1/APC Isotype control (#ANC-278-060). Binding was blocked when beads were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD152 antibody (#ANC-359-020).
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