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Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-CTNNA1 Antibody

HPA046119
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCTNNA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CTNNA1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1495
  • Target name
    CTNNA1
  • Target description
    catenin alpha 1
  • Target synonyms
    CAP102, MDBS2, MDPT2, catenin alpha-1, alpha-E-catenin, catenin (cadherin-associated protein), alpha 1, 102kDa, epididymis secretory sperm binding protein, renal carcinoma antigen NY-REN-13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35221
  • Protein Name
    Catenin alpha-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CTNNA1 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 antigen affinity purified polyclonal antibody (Catalog # PB9137) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 47KD. The expected band size for CTNNA1 is at 47KD.
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