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Anti-CXCR1 Antibody

144-64333
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCXCR1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CXCR1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID3577
  • Target name
    CXCR1
  • Target description
    C-X-C motif chemokine receptor 1
  • Target synonyms
    C-C, C-C-CKR-1, CD128, CD181, CDw128a, CKR-1, CMKAR1, IL8R1, IL8RA, IL8RBA, C-X-C chemokine receptor type 1, CXC-R1, CXCR-1, IL-8 receptor type 1, IL-8R A, chemokine (C-X-C motif) receptor 1, high affinity interleukin-8 receptor A, interleukin 8 receptor, alpha, interleukin-8 receptor type 1, interleukin-8 receptor type A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25024
  • Protein Name
    C-X-C chemokine receptor type 1
  • Scientific Description
    CXCR1 pAb
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CXCR1 using anti-CXCR1 antibody (PA2080). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR1 antigen affinity purified polyclonal antibody (Catalog # PA2080) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR1 at approximately 50 kDa. The expected band size for CXCR1 is at 40 kDa.
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