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Immunohistochemical staining of human bone marrow shows positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows positivity in hematopoietic cells.

Anti-CXCR1 Antibody

HPA031991
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetCXCR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CXCR1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3577
  • Target name
    CXCR1
  • Target description
    C-X-C motif chemokine receptor 1
  • Target synonyms
    C-C; C-C-CKR-1; CD128; CD181; CDw128a; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXC-R1; CXCR-1; high affinity interleukin-8 receptor A; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25024
  • Protein Name
    C-X-C chemokine receptor type 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CXCR1 using anti-CXCR1 antibody (PA2080). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR1 antigen affinity purified polyclonal antibody (Catalog # PA2080) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR1 at approximately 50 kDa. The expected band size for CXCR1 is at 40 kDa.
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