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Immunohistochemical staining of human skeletal muscle shows moderate granular positivity in cytoplasm in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate granular positivity in cytoplasm in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate granular positivity in cytoplasm in myocytes.

Anti-CYC1 Antibody

HPA001247
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCYC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CYC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1537
  • Target name
    CYC1
  • Target description
    cytochrome c1
  • Target synonyms
    complex III subunit 4; complex III subunit IV; cytochrome b-c1 complex subunit 4; cytochrome c-1; cytochrome c1, heme protein, mitochondrial; MC3DN6; ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; UQCR4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08574
  • Protein Name
    Cytochrome c1, heme protein, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYC1 using anti-CYC1 antibody (A02958-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYC1 antigen affinity purified polyclonal antibody (Catalog # A02958-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYC1 at approximately 33 kDa. The expected band size for CYC1 is at 35 kDa.
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