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Western Blot analysis of rat-musle cells using Cytochrome c1 Polyclonal Antibody
Western Blot analysis of rat-musle cells using Cytochrome c1 Polyclonal Antibody
Western Blot analysis of rat-musle cells using Cytochrome c1 Polyclonal Antibody

CYC1 Antibody

CSB-PA002014
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCYC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1537
  • Target name
    CYC1
  • Target description
    cytochrome c1
  • Target synonyms
    complex III subunit 4; complex III subunit IV; cytochrome b-c1 complex subunit 4; cytochrome c-1; cytochrome c1, heme protein, mitochondrial; MC3DN6; ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; UQCR4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08574
  • Protein Name
    Cytochrome c1, heme protein, mitochondrial
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYC1 using anti-CYC1 antibody (A02958-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYC1 antigen affinity purified polyclonal antibody (Catalog # A02958-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYC1 at approximately 33 kDa. The expected band size for CYC1 is at 35 kDa.
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Immunohistochemical staining of human skeletal muscle shows moderate granular positivity in cytoplasm in myocytes.
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