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Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.

Anti-CYP11B1 Antibody

HPA056348
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCYP11B1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CYP11B1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1584
  • Target name
    CYP11B1
  • Target description
    cytochrome P450 family 11 subfamily B member 1
  • Target synonyms
    CPN1; CYP11B; cytochrome P450 11B1, mitochondrial; cytochrome p450 XIB1; cytochrome P450, family 11, subfamily B, polypeptide 1; cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 1; cytochrome P450C11; cytochrome P-450c11; FHI; P450C11; steroid 11-beta-hydroxylase, CYP11B1; steroid 11-beta-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15538
  • Protein Name
    Cytochrome P450 11B1, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP11B1/C11B2/CYP11B2 using anti-CYP11B1/C11B2/CYP11B2 antibody (A03766-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP11B1/C11B2/CYP11B2 antigen affinity purified polyclonal antibody (Catalog # A03766-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP11B1/C11B2/CYP11B2 at approximately 55KD. The expected band size for CYP11B1/C11B2/CYP11B2 is at 55KD.
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