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Western Blot analysis of COLO205 cells using CYP11B1/2 Polyclonal Antibody
Western Blot analysis of COLO205 cells using CYP11B1/2 Polyclonal Antibody
Western Blot analysis of COLO205 cells using CYP11B1/2 Polyclonal Antibody

CYP11B1/CYP11B2 Antibody

CSB-PA001924
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCYP11B1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP11B1/CYP11B2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1584
  • Target name
    CYP11B1
  • Target description
    cytochrome P450 family 11 subfamily B member 1
  • Target synonyms
    CPN1, CYP11B, FHI, P450C11, cytochrome P450 11B1, mitochondrial, cytochrome P-450c11, cytochrome P450, family 11, subfamily B, polypeptide 1, cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 1, cytochrome P450C11, cytochrome p450 XIB1, steroid 11-beta-hydroxylase, CYP11B1, steroid 11-beta-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15538
  • Protein Name
    Cytochrome P450 11B1, mitochondrial
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP11B1/C11B2/CYP11B2 using anti-CYP11B1/C11B2/CYP11B2 antibody (A03766-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP11B1/C11B2/CYP11B2 antigen affinity purified polyclonal antibody (Catalog # A03766-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP11B1/C11B2/CYP11B2 at approximately 55KD. The expected band size for CYP11B1/C11B2/CYP11B2 is at 55KD.
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