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Anti-Cystatin C [LC01], Human IgG1, Fc Silent(tm), kappa

AB04698-10.3
Absolute Antibody
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetCST3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Cystatin C [LC01], Human IgG1, Fc Silent(tm), kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding reactivity of this antibody for human cystatin C was determined using ELISA. This antibody can be used for the analysis of cystatin C presence in serum samples using latex turbidimetry (CN116535513).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LC01
  • Gene ID1471
  • Target name
    CST3
  • Target description
    cystatin C
  • Target synonyms
    ADLDWA, ARMD11, HEL-S-2, cystatin-C, bA218C14.4 (cystatin C), cystatin 3, epididymis secretory protein Li 2, gamma-trace, neuroendocrine basic polypeptide, post-gamma-globulin
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP01034
  • Protein Name
    Cystatin-C
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.
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