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Western Blot analysis of 293T cells using Cystatin C Polyclonal Antibody
Western Blot analysis of 293T cells using Cystatin C Polyclonal Antibody
Western Blot analysis of 293T cells using Cystatin C Polyclonal Antibody

CST3 Antibody

CSB-PA002010
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetCST3
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Overview

  • Supplier
    Cusabio
  • Product Name
    CST3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1471
  • Target name
    CST3
  • Target description
    cystatin C
  • Target synonyms
    ADLDWA, ARMD11, HEL-S-2, cystatin-C, bA218C14.4 (cystatin C), cystatin 3, epididymis secretory protein Li 2, gamma-trace, neuroendocrine basic polypeptide, post-gamma-globulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01034
  • Protein Name
    Cystatin-C
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.
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