
Figure 1. Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.
Anti-Cystatin C/CST3 Antibody Picoband(r)
A00961-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCST3
Overview
- SupplierBoster Bio
- Product NameAnti-Cystatin C/CST3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID1471
- Target nameCST3
- Target descriptioncystatin C
- Target synonymsARMD11; bA218C14.4 (cystatin C); cystatin 3; cystatin-C; epididymis secretory protein Li 2; gamma-trace; HEL-S-2; neuroendocrine basic polypeptide; post-gamma-globulin
- HostRabbit
- IsotypeIgG
- Protein IDP01034
- Protein NameCystatin-C
- Scientific DescriptionBoster Bio Anti-Cystatin C/CST3 Antibody Picoband® catalog # A00961-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203