
Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoskeleton by immunohistochemical analysis. Sample: Paraffin-embedded dog malignant carcinoma. Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cytokeratin 18 antibody [N2C2], Internal
GTX105624
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse
TargetKRT18
Overview
- SupplierGeneTex
- Product NameCytokeratin 18 antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.54 mg/ml
- ConjugateUnconjugated
- Gene ID3875
- Target nameKRT18
- Target descriptionkeratin 18
- Target synonymsCK-18, CYK18, K18, keratin, type I cytoskeletal 18, cell proliferation-inducing gene 46 protein, cytokeratin 18, keratin 18, type I
- HostRabbit
- IsotypeIgG
- Protein IDP05783
- Protein NameKeratin, type I cytoskeletal 18
- Scientific DescriptionKRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
- ReactivityCanine, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wuputra K, Hsiao PJ, Chang WT, et al. FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells. Int J Mol Sci. 2022,23(14). doi: 10.3390/ijms23147782Read this paper
- Yu HI, Chou HC, Su YC, et al. Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells. J Pharm Biomed Anal. 2018,160:344-350. doi: 10.1016/j.jpba.2018.08.008Read this paper
- Luni C, Giulitti S, Serena E, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016,13(5):446-52. doi: 10.1038/nmeth.3832Read this paper
- Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012Read this paper
- Xing C, Ci X, Sun X, et al. Klf5 deletion promotes Pten deletion-initiated luminal-type mouse prostate tumors through multiple oncogenic signaling pathways. Neoplasia. 2014,16(11):883-99. doi: 10.1016/j.neo.2014.09.006Read this paper






![Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm. Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX105624/GTX105624_39890_IFA_w_23060120_323.webp)
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000. Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX105624/GTX105624_39890_20161006_WB_shRNA_watermark_w_23060120_237.webp)



![FACS analysis of HeLa cells using GTX02657 Cytokeratin 18 antibody [KRT18/2808R]. Blue : Primary antibody Red : Isotype control](https://www.genetex.com/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_FACS_w_23053122_467.webp)
![IHC-P analysis of human colon carcinoma section using GTX02658 Cytokeratin 18 antibody [KRT18/2819R].](https://www.genetex.com/upload/website/prouct_img/normal/GTX02658/GTX02658_20210319_IHC-P_w_23053122_304.webp)
![IHC-P analysis of human gastric antrum tissue using GTX04374 Cytokeratin 18 antibody [MSVA-118R] HistoMAX?. Strong cytokeratin 18 staining of all epithelial cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04374/GTX04374_20230728_IHC-P_39_23072722_592.webp)


![IHC-P analysis of human colon tissue using GTX57172 Cytokeratin 18 antibody [IHC018] (10X)](https://www.genetex.com/upload/website/prouct_img/normal/GTX57172/GTX57172_20180619_IHC-P_w_23061123_313.webp)