Anti-Cytokeratin 18 [RGE53]

Absolute Antibody

Anti-Cytokeratin 18 [RGE53]

9

The antibody reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.

The antibody recognized glandular epithelial cells from female breast, digestive, respiratory, and urogenital tracts, endocrine and exocrine tissues, and mesothelial cells. Further, the antibody could distinguish adenocarcinomas and mesotheliomas, which stain positively in the indirect immunofluorescence technique (Ramaekers et al., 1983; PMID: 6193333). Further, the antibody produced fibrillar staining patterns in cultured HeLa cells, in cultured human hepatoma cells, F9 mouse teratocarcinoma cells and in a human colon carcinoma cell line (WiDr-218). The antibody detected Cytokeratin 18 in HeLa cells and cultured human hepatoma cells by western blot analysis (Ramaekers et al., 1983; PMID: 6350235). The antibody reacted with glandular and ductal epithelia, including those of the gastrointestinal tract, prostate, and kidney and with hepatocytes and mesothelium in immunohistochemistry (Ramaekers et al., 1990; PMID: 1690512).

Flow Cytometry, Western Blot, ImmunoHistoChemistry

Research Use Only

Monoclonal

RGE53

KRT18

keratin 18

cell proliferation-inducing gene 46 protein; CK-18; CYK18; cytokeratin 18; K18; keratin 18, type I; keratin, type I cytoskeletal 18

Mouse

IgG1

Keratin, type I cytoskeletal 18

Canine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish

-20°C,2°C to 8°C

12352203