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ProductsBack/Anti-Cytokeratin 5 and 8 [RCK102]

Anti-Cytokeratin 5 and 8 [RCK102]

AB03335-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Feline, Hamster, Human, Mouse, Porcine, Rabbit, Rat
TargetKRT5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Cytokeratin 5 and 8 [RCK102]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody recognized keratins 5 and 8, and as a result stained virtually all epithelial tissues. This antibody was used for immunohistochemistry on classic SCLC as well as the adenocarcinoma cell lines (Broers et al., 1986; PMID: 2433295). Further, endocervical columnar cells, cervical immature squamous metaplasia, cervical CIN I, CIN II and CIN III reacted with the antibody in immunohistochemistry (Smedts et al., 1990; PMID: 1690513). The antibody detected keratins 5 and 8 by western blot analysis on T 24, HeLa and KB cells (Ramaekers et al., 1987; PMID: 2436934).
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RCK102
  • Gene ID3852
  • Target name
    KRT5
  • Target description
    keratin 5
  • Target synonyms
    CK5, DDD, DDD1, EBS1, EBS2, EBS2A, EBS2B, EBS2C, EBS2D, EBS2E, EBS2F, K5, KRT5A, keratin, type II cytoskeletal 5, 58 kda cytokeratin, CK-5, cytokeratin-5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), keratin 5, type II, type-II keratin Kb5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05787
  • Protein Name
    Keratin, type II cytoskeletal 8
  • Reactivity
    Canine, Feline, Hamster, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (A00398). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 5 antigen affinity purified polyclonal antibody (Catalog # A00398) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 5 at approximately 62 kDa. The expected band size for Cytokeratin 5 is at 62 kDa.
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