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Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & nuclear bodies.
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & nuclear bodies.
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & nuclear bodies.

Anti-DAXX Antibody

HPA065779
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDAXX
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DAXX
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1616
  • Target name
    DAXX
  • Target description
    death domain associated protein
  • Target synonyms
    BING2, DAP6, EAP1, SMIM40, death domain-associated protein 6, CENP-C binding protein, ETS1-associated protein 1, Fas-binding protein, death-associated protein 6, fas death domain-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UER7
  • Protein Name
    Death domain-associated protein 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Daxx using anti-Daxx antibody (PB9550). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Daxx antigen affinity purified polyclonal antibody (Catalog # PB9550) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Daxx at approximately 115 kDa. The expected band size for Daxx is at 81 kDa.
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Immunohistochemical staining of human cervix shows moderate nuclear immunoreactivity in epithelial and underlying connective tissue cells.
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