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Western Blot analysis of HuvEc cells using Daxx Polyclonal Antibody
Western Blot analysis of HuvEc cells using Daxx Polyclonal Antibody
Western Blot analysis of HuvEc cells using Daxx Polyclonal Antibody

DAXX Antibody

CSB-PA002077
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDAXX
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Overview

  • Supplier
    Cusabio
  • Product Name
    DAXX Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1616
  • Target name
    DAXX
  • Target description
    death domain associated protein
  • Target synonyms
    BING2; CENP-C binding protein; DAP6; death domain-associated protein 6; death-associated protein 6; EAP1; ETS1-associated protein 1; fas death domain-associated protein; Fas-binding protein; SMIM40
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UER7
  • Protein Name
    Death domain-associated protein 6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Daxx using anti-Daxx antibody (PB9550). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Daxx antigen affinity purified polyclonal antibody (Catalog # PB9550) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Daxx at approximately 115 kDa. The expected band size for Daxx is at 81 kDa.
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