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Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Anti-DDT Antibody

HPA049871
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDDT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DDT
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1652
  • Target name
    DDT
  • Target description
    D-dopachrome tautomerase
  • Target synonyms
    D-DT, DDCT, MIF-2, MIF2, D-dopachrome decarboxylase, phenylpyruvate tautomerase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30046
  • Protein Name
    D-dopachrome decarboxylase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDT using anti-DDT antibody (A01354). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human liver tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # A01354) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 13 kDa. The expected band size for DDT is at 13 kDa.
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