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Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200

DDT Antibody

CSB-PA221047
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDDT
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Overview

  • Supplier
    Cusabio
  • Product Name
    DDT Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1652
  • Target name
    DDT
  • Target description
    D-dopachrome tautomerase
  • Target synonyms
    DDCT; D-dopachrome decarboxylase; D-DT; MIF2; MIF-2; phenylpyruvate tautomerase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30046
  • Protein Name
    D-dopachrome decarboxylase
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDT using anti-DDT antibody (A01354). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human liver tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # A01354) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 13 kDa. The expected band size for DDT is at 13 kDa.
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