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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Anti-DEFA1 Antibody

HPA052517
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDEFA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DEFA1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1667
  • Target name
    DEFA1
  • Target description
    defensin alpha 1
  • Target synonyms
    DEF1, DEFA2, HNP-1, HP-1, HP1, MRS, neutrophil defensin 1, defensin, alpha 1, myeloid-related sequence, defensin, alpha 2, myeloid-related sequence
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP59665
  • Protein Name
    Neutrophil defensin 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Alpha Defensin 1 using anti-Alpha Defensin 1 antibody (A10546). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat testis tissue lysate, lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Defensin 1 antigen affinity purified polyclonal antibody (Catalog # A10546) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Defensin 1 at approximately 19KD. The expected band size for Alpha Defensin 1 is at 10KD.
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