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ProductsBack/DEFA1 / Defensin Alpha 1 Antibody

DEFA1 / Defensin Alpha 1 Antibody

LS-C402237
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDEFA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DEFA1 / Defensin Alpha 1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:50), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1667
  • Target name
    DEFA1
  • Target description
    defensin alpha 1
  • Target synonyms
    DEF1, DEFA2, HNP-1, HP-1, HP1, MRS, neutrophil defensin 1, defensin, alpha 1, myeloid-related sequence, defensin, alpha 2, myeloid-related sequence
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alpha Defensin 1 using anti-Alpha Defensin 1 antibody (A10546). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat testis tissue lysate, lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Defensin 1 antigen affinity purified polyclonal antibody (Catalog # A10546) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Defensin 1 at approximately 19KD. The expected band size for Alpha Defensin 1 is at 10KD.
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