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Anti-Desmin [14G1C12]

Ab03233-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDES
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Desmin [14G1C12]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody was used for immunohistochemistry on esophageal, leiomyoma and rhabdomyosarcoma tissue (CN109485724).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14G1C12
  • Gene ID1674
  • Target name
    DES
  • Target description
    desmin
  • Target synonyms
    cardiomyopathy, dilated 1F (autosomal dominant); CDCD3; CSM1; CSM2; desmin; epididymis secretory sperm binding protein; intermediate filament protein; LGMD1D; LGMD1E; LGMD2R
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17661
  • Protein Name
    Desmin
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing BALB/c mice with recombinant DESMIN.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Desmin using anti-Desmin antibody (PB9105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse C2C12 whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmin antigen affinity purified polyclonal antibody (Catalog # PB9105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.
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