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Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Desmin Polyclonal Antibody

BS-1026R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDES
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Overview

  • Supplier
    Bioss
  • Product Name
    Desmin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1674
  • Target name
    DES
  • Target description
    desmin
  • Target synonyms
    CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R, desmin, cardiomyopathy, dilated 1F (autosomal dominant), cardiomyopathy, dilated 1I, epididymis secretory sperm binding protein, intermediate filament protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17661
  • Protein Name
    Desmin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ding F, Shan C, Li H, et al. Simvastatin alleviated diabetes mellitus-induced erectile dysfunction in rats by enhancing AMPK pathway-induced autophagy. Andrology. 2020,8(3):780-792. doi: 10.1111/andr.12758
    Read this paper
  • Yin X, Li Q, Wei H, et al. Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Arch Biochem Biophys. 2019,669:1-10. doi: 10.1016/j.abb.2019.05.016
    Read this paper
  • Li T, Zhang G, Wu P, et al. Dissection of Myogenic Differentiation Signatures in Chickens by RNA-Seq Analysis. Genes (Basel). 2018,9(1). doi: 10.3390/genes9010034
    Read this paper
  • Li Y, Xiong L, Gong J. Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells. Am J Transl Res. 2017,9(6):2865-2877.
    Read this paper
  • Liu Y, Nelson T, Cromeens B, et al. HB-EGF embedded in PGA/PLLA scaffolds via subcritical CO2 augments the production of tissue engineered intestine. Biomaterials. 2016,103:150-159. doi: 10.1016/j.biomaterials.2016.06.039
    Read this paper
  • Ruan ZB, Zhu L, Yin YG, et al. Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells. Cytotechnology. 2016,68(4):1257-65. doi: 10.1007/s10616-015-9886-5
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Desmin using anti-Desmin antibody (PB9105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse C2C12 whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmin antigen affinity purified polyclonal antibody (Catalog # PB9105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.
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