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Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Desmin Polyclonal Antibody

BS-1026R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDES
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Desmin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1674
  • Target name
    DES
  • Target description
    desmin
  • Target synonyms
    cardiomyopathy, dilated 1F (autosomal dominant); CDCD3; CSM1; CSM2; desmin; epididymis secretory sperm binding protein; intermediate filament protein; LGMD1D; LGMD1E; LGMD2R
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17661
  • Protein Name
    Desmin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Simvastatin alleviated diabetes mellitus-induced erectile dysfunction in rats by enhancing AMPK pathway-induced autophagy. Ding F et al., 2020 May, Andrology
    Read more
  • Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Yin X et al., 2019 Jul 15, Arch Biochem Biophys
    Read more
  • Dissection of Myogenic Differentiation Signatures in Chickens by RNA-Seq Analysis. Li T et al., 2018 Jan 11, Genes (Basel)
    Read more
  • Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells. Li Y et al., 2017, Am J Transl Res
    Read more
  • HB-EGF embedded in PGA/PLLA scaffolds via subcritical CO2 augments the production of tissue engineered intestine. Liu Y et al., 2016 Oct, Biomaterials
    Read more
  • Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells. Ruan ZB et al., 2016 Aug, Cytotechnology
    Read more

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Figure 1. Western blot analysis of Desmin using anti-Desmin antibody (PB9105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse C2C12 whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmin antigen affinity purified polyclonal antibody (Catalog # PB9105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.
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