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Anti-desmoglein-3 [5G11]

Ab01659-1.1
Absolute Antibody
ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetDSG3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-desmoglein-3 [5G11]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for detection and analysis of desmoglein-3 via various methods. For instance, 5G11 was utilized todetect desmoglein-3 in human scalp hair follicles and epidermal inclusions via immunohistochemistry (Wu et al., 2003). Another group successfully used this antibody to identify desmoglein-3 via Western blot (Mao et al., 2017; pmid: 28469076). The antibody 5G11 was also used to detect DSG3 in cells via immunofluorescence (Chen et al., 2013; pmid: 23737966).
  • Applications
    Dot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; FC; IF; IHC; dot blot; IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5G11
  • Gene ID1830
  • Target name
    DSG3
  • Target description
    desmoglein 3
  • Target synonyms
    ABOLM, CDHF6, PVA, desmoglein-3, 130 kDa pemphigus vulgaris antigen, cadherin family member 6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP32926
  • Protein Name
    Desmoglein-3
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunizing Balb/c mice with a recombinant protein corresponding to human desmoglein-3.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Desmoglein 3/PVA/DSG3 using anti-Desmoglein 3/PVA/DSG3 antibody (A03093). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoglein 3/PVA/DSG3 antigen affinity purified polyclonal antibody (Catalog # A03093) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoglein 3/PVA/DSG3 at approximately 130 kDa. The expected band size for Desmoglein 3/PVA/DSG3 is at 130 kDa.
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