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ProductsBack/Desmoglein 3 antibody
Immunoprecipitation of DSG3 protein from A431 whole cell extracts using 5 μg of DSG3 antibody (GTX129931). Western blot analysis was performed using DSG3 antibody (GTX129931). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of DSG3 protein from A431 whole cell extracts using 5 μg of DSG3 antibody (GTX129931). Western blot analysis was performed using DSG3 antibody (GTX129931). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of DSG3 protein from A431 whole cell extracts using 5 μg of DSG3 antibody (GTX129931). Western blot analysis was performed using DSG3 antibody (GTX129931). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Desmoglein 3 antibody

GTX129931
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetDSG3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Desmoglein 3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.38 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1830
  • Target name
    DSG3
  • Target description
    desmoglein 3
  • Target synonyms
    ABOLM, CDHF6, PVA, desmoglein-3, 130 kDa pemphigus vulgaris antigen, cadherin family member 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32926
  • Protein Name
    Desmoglein-3
  • Scientific Description
    Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Desmoglein 3/PVA/DSG3 using anti-Desmoglein 3/PVA/DSG3 antibody (A03093). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoglein 3/PVA/DSG3 antigen affinity purified polyclonal antibody (Catalog # A03093) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoglein 3/PVA/DSG3 at approximately 130 kDa. The expected band size for Desmoglein 3/PVA/DSG3 is at 130 kDa.
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