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Anti-DIAPH1 Antibody

144-05772
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetDIAPH1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-DIAPH1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1729
  • Target name
    DIAPH1
  • Target description
    diaphanous related formin 1
  • Target synonyms
    DFNA1; DIA1; DRF1; hDIA1; LFHL1; protein diaphanous homolog 1; SCBMS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60610
  • Protein Name
    Protein diaphanous homolog 1
  • Scientific Description
    DIAPH1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DIAPH1 using anti-DIAPH1 antibody (A02308-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIAPH1 antigen affinity purified polyclonal antibody (Catalog # A02308-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIAPH1 at approximately 150 kDa. The expected band size for DIAPH1 is at 150 kDa.
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