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Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.

Anti-DLL4 Antibody

HPA023392
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetDLL4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DLL4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54567
  • Target name
    DLL4
  • Target description
    delta like canonical Notch ligand 4
  • Target synonyms
    AOS6; delta 4; delta ligand 4; delta4; delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; drosophila Delta homolog 4; hdelta2; notch ligand delta-2; notch ligand DLL4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR61
  • Protein Name
    Delta-like protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80 kDa. The expected band size for DLL4 is at 75 kDa.
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