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Immunohistochemical staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate membranous positivity in myocytes.

Anti-DMD Antibody

HPA002725
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDMD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DMD
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1756
  • Target name
    DMD
  • Target description
    dystrophin
  • Target synonyms
    BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85, dystrophin, mutant dystrophin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Dystrophin using anti-Dystrophin antibody (PB9276). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dystrophin antigen affinity purified polyclonal antibody (Catalog # PB9276) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dystrophin at approximately 427KD. The expected band size for Dystrophin is at 427KD.
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