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Anti-DNAJC3 (Center) Antibody

102-25313
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetDNAJC3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-DNAJC3 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB57780
  • Gene ID5611
  • Target name
    DNAJC3
  • Target description
    DnaJ heat shock protein family (Hsp40) member C3
  • Target synonyms
    ACPHD, ERdj6, HP58, P58, P58IPK, PRKRI, p58(IPK), dnaJ homolog subfamily C member 3, DnaJ (Hsp40) homolog, subfamily C, member 3, ER-resident protein ERdj6, endoplasmic reticulum DNA J domain-containing protein 6, interferon-induced, double-stranded RNA-activated protein kinase inhibitor, protein kinase inhibitor of 58 kDa, protein kinase inhibitor p58, protein-kinase, interferon-inducible double stranded RNA dependent inhibitor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13217
  • Protein Name
    DnaJ homolog subfamily C member 3
  • Scientific Description
    Rabbit Anti-DNAJC3 (Center) Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNAJC3 using anti-DNAJC3 antibody (A04805-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse pancreas tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC3 antigen affinity purified polyclonal antibody (Catalog # A04805-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC3 at approximately 58 kDa. The expected band size for DNAJC3 is at 58 kDa.
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