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Immunohistochemical staining of human thyroid gland shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows cytoplasmic positivity in glandular cells.

Anti-DNAJC3 Antibody

HPA039336
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDNAJC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DNAJC3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5611
  • Target name
    DNAJC3
  • Target description
    DnaJ heat shock protein family (Hsp40) member C3
  • Target synonyms
    ACPHD, ERdj6, HP58, P58, P58IPK, PRKRI, p58(IPK), dnaJ homolog subfamily C member 3, DnaJ (Hsp40) homolog, subfamily C, member 3, ER-resident protein ERdj6, endoplasmic reticulum DNA J domain-containing protein 6, interferon-induced, double-stranded RNA-activated protein kinase inhibitor, protein kinase inhibitor of 58 kDa, protein kinase inhibitor p58, protein-kinase, interferon-inducible double stranded RNA dependent inhibitor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13217
  • Protein Name
    DnaJ homolog subfamily C member 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNAJC3 using anti-DNAJC3 antibody (A04805-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse pancreas tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC3 antigen affinity purified polyclonal antibody (Catalog # A04805-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC3 at approximately 58 kDa. The expected band size for DNAJC3 is at 58 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA109707(DNAJC3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
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