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Anti-Dnmt1 Antibody

Anti-Dnmt1 Antibody

A84464
Antibodies.com
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Dnmt1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to Dnmt1.
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dnmt1 using anti-Dnmt1 antibody (A00172-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-937 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt1 antigen affinity purified polyclonal antibody (Catalog # A00172-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dnmt1 at approximately 200KD. The expected band size for Dnmt1 is at 183KD.
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