Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.
![DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_40135_20150612_IHC_w_23060519_627.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20210129_IHC-P_w_23060519_304.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20201225_IHC-P_M_w_23060519_444.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20170330_WB_w_23060519_224.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011) 5 % SDS-PAGE The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_40135_IP_w_23060519_118.webp "DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011) 5 % SDS-PAGE The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20170503_IFA_w_23060519_934.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20180316_WB_KO_watermark_w_23060519_129.webp "Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.
DNMT1 antibody [N1], N-term
GTX116011
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetDNMT1
Overview
- SupplierGeneTex
- Product NameDNMT1 antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.18 mg/ml
- ConjugateUnconjugated
- Gene ID1786
- Target nameDNMT1
- Target descriptionDNA methyltransferase 1
- Target synonymsADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA MTase HsaI, DNA methyltransferase HsaI
- HostRabbit
- IsotypeIgG
- Protein IDP26358
- Protein NameDNA (cytosine-5)-methyltransferase 1
- Scientific DescriptionDNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhao Z, Li J, Feng Y, et al. Host DNA Demethylation Induced by DNMT1 Inhibition Up-Regulates Antiviral OASL Protein during Influenza a Virus Infection. Viruses. 2023,15(8). doi: 10.3390/v15081646Read this paper
- Tu H, Zhang Z, Li J, et al. Loss of MEG3 contributes to the enhanced migration and invasion in arsenic-induced carcinogenesis through NQO1/FSCN1 pathway. Am J Cancer Res. 2023,13(6):2307-2322.Read this paper
- Wu YH, Huang YF, Wu PY, et al. The downregulation of miR-509-3p expression by collagen type XI alpha 1-regulated hypermethylation facilitates cancer progression and chemoresistance via the DNA methyltransferase 1/Small ubiquitin-like modifier-3 axis in ovarian cancer cells. J Ovarian Res. 2023,16(1):124. doi: 10.1186/s13048-023-01191-5Read this paper
- Zhang N, Gao X, Yuan Q, et al. E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1. Clin Epigenetics. 2023,15(1):77. doi: 10.1186/s13148-023-01492-yRead this paper
- Wu YH, Huang YF, Wu PY, et al. The Downregulation of miR-509-3p Expression by Collagen Type XI Alpha 1-Regulated Hypermethylation Facilitates Cancer Progression and Chemoresistance via the DNA Methyltransferase 1/Small Ubiquitin-like Modifier-3 Axis in Ovarian Cancer Cells. Res Sq. 2023,:pii: rs.3.rs-2592453. doi: 10.21203/rs.3.rs-2592453/v1.Read this paper
- Wang Q, Li G, Ma X, et al. LncRNA TINCR impairs the efficacy of immunotherapy against breast cancer by recruiting DNMT1 and downregulating MiR-199a-5p via the STAT1-TINCR-USP20-PD-L1 axis. Cell Death Dis. 2023,14(2):76. doi: 10.1038/s41419-023-05609-2Read this paper
- Kawai T, Richards JS, Shimada M. Large-scale DNA demethylation occurs in proliferating ovarian granulosa cells during mouse follicular development. Commun Biol. 2021,4(1):1334. doi: 10.1038/s42003-021-02849-wRead this paper
- Tessier SN, Ingelson-Filpula WA, Storey KB. Epigenetic regulation by DNA methyltransferases during torpor in the thirteen-lined ground squirrel Ictidomys tridecemlineatus. Mol Cell Biochem. 2021,476(11):3975-3985. doi: 10.1007/s11010-021-04214-1Read this paper
- Wang Q, Liu J, You Z, et al. LncRNA TINCR favors tumorigenesis via STAT3-TINCR-EGFR-feedback loop by recruiting DNMT1 and acting as a competing endogenous RNA in human breast cancer. Cell Death Dis. 2021,12(1):83. doi: 10.1038/s41419-020-03188-0Read this paper
- Zhang D, An X, Li Q, et al. Thioguanine Induces Apoptosis in Triple-Negative Breast Cancer by Regulating PI3K-AKT Pathway. Front Oncol. 2020,10:524922. doi: 10.3389/fonc.2020.524922Read this paper
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