Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.
![DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_40135_20150612_IHC_w_23060519_627.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20210129_IHC-P_w_23060519_304.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20201225_IHC-P_M_w_23060519_444.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20170330_WB_w_23060519_224.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011) 5 % SDS-PAGE The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_40135_IP_w_23060519_118.webp "DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011) 5 % SDS-PAGE The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20170503_IFA_w_23060519_934.webp "DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116011/GTX116011_42732_20180316_WB_KO_watermark_w_23060519_129.webp "Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.
DNMT1 antibody [N1], N-term
GTX116011
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetDNMT1
Overview
- SupplierGeneTex
- Product NameDNMT1 antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.18 mg/ml
- ConjugateUnconjugated
- Gene ID1786
- Target nameDNMT1
- Target descriptionDNA methyltransferase 1
- Target synonymsADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA MTase HsaI, DNA methyltransferase HsaI
- HostRabbit
- IsotypeIgG
- Protein IDP26358
- Protein NameDNA (cytosine-5)-methyltransferase 1
- Scientific DescriptionDNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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