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Anti-DNMT3B Polyclonal Antibody

Anti-DNMT3B Polyclonal Antibody

25219
BPS Bioscience
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetDNMT3B
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    Anti-DNMT3B Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ImmunoFluorescence; Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1789
  • Target name
    DNMT3B
  • Target description
    DNA methyltransferase 3 beta
  • Target synonyms
    FSHD4, ICF, ICF1, M.HsaIIIB, DNA (cytosine-5)-methyltransferase 3B, DNA (cytosine-5-)-methyltransferase 3 beta, DNA MTase HsaIIIB, DNA cytosine-5--methyltransferase 3 beta, DNA methyltransferase HsaIIIB
  • Protein IDQ9UBC3
  • Protein Name
    DNA (cytosine-5)-methyltransferase 3B
  • Scientific Description
    Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using 3 KLHconjugated synthetic peptides containing sequences from different parts of the protein.
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNMT3B using anti-DNMT3B antibody (A00319-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B antigen affinity purified polyclonal antibody (Catalog # A00319-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3B at approximately 96 kDa. The expected band size for DNMT3B is at 96 kDa.
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