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Formalin-fixed and paraffin embedded: human colon cancer tissue labeled with Anti-Dnmt-3-beta Polyclonal Antibody, Unconjugated (bs-0301R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: human colon cancer tissue labeled with Anti-Dnmt-3-beta Polyclonal Antibody, Unconjugated (bs-0301R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: human colon cancer tissue labeled with Anti-Dnmt-3-beta Polyclonal Antibody, Unconjugated (bs-0301R) at 1:600, followed by conjugation to the secondary antibody and DAB staining

Dnmt3b Polyclonal Antibody

BS-0301R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDNMT3B
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Overview

  • Supplier
    Bioss
  • Product Name
    Dnmt3b Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1789
  • Target name
    DNMT3B
  • Target description
    DNA methyltransferase 3 beta
  • Target synonyms
    FSHD4, ICF, ICF1, M.HsaIIIB, DNA (cytosine-5)-methyltransferase 3B, DNA (cytosine-5-)-methyltransferase 3 beta, DNA MTase HsaIIIB, DNA cytosine-5--methyltransferase 3 beta, DNA methyltransferase HsaIIIB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBC3
  • Protein Name
    DNA (cytosine-5)-methyltransferase 3B
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wu Y, Bu F, Yu H, et al. Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis. Toxicol Appl Pharmacol. 2017,315:35-49. doi: 10.1016/j.taap.2016.12.002
    Read this paper
  • Huang C, Liu H, Gong XL, et al. Expression of DNA methyltransferases and target microRNAs in human tissue samples related to sporadic colorectal cancer. Oncol Rep. 2016,36(5):2705-2714. doi: 10.3892/or.2016.5104
    Read this paper

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Figure 1. Western blot analysis of DNMT3B using anti-DNMT3B antibody (A00319-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B antigen affinity purified polyclonal antibody (Catalog # A00319-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3B at approximately 96 kDa. The expected band size for DNMT3B is at 96 kDa.
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