Anti-Do (b) [Mima-73]

Absolute Antibody

Anti-Do (b) [Mima-73]

9

The specificity of this antibody has been confirmed in haemagglutination studies, where it reacts with Do(a-b+) and Do(a+b+) RBCs, but not with Do(a+b-) RBCs (Grodecka et al, 2012). The epitope recognised by this antibody is resistant to treatment with papain, alpha-chymotrypsin and neuraminidase, and sensitive to treatment with trypsin and DTT (Grodecka et al, 2012). This antibody has been used, as part of a panel of antibodies specific for different epitopes on the Dombrock glycoprotein, in flow cytometric and haemagglutination analysis of human red blood cells (Mayer et al, 2010; Costa et al, 2010).

Flow Cytometry

Research Use Only

Monoclonal

Mima-73

ART4

ADP-ribosyltransferase 4 (inactive) (Dombrock blood group)

ARTC4, CD297, DO, DO/ART4, DOK1, ecto-ADP-ribosyltransferase 4, ADP-ribosyltransferase 4 (DO blood group), ADP-ribosyltransferase 4 (Dombrock blood group), ADP-ribosyltransferase C2 and C3 toxin-like 4, Do glycoprotein, Dombrock blood group carrier molecule, Dombrock glycoprotein, NAD(+)--protein-arginine ADP-ribosyltransferase 4, NAD(P)(+)--arginine ADP-ribosyltransferase 4, mono-ADP-ribosyltransferase 4

Rabbit

IgG

Ecto-ADP-ribosyltransferase 4

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2c format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203