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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.

Anti-E2F4 Antibody

HPA054128
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetE2F4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-E2F4
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1874
  • Target name
    E2F4
  • Target description
    E2F transcription factor 4
  • Target synonyms
    E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; transcription factor E2F4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16254
  • Protein Name
    Transcription factor E2F4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of E2F4 using anti-E2F4 antibody (PB10057). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: U20S whole cell lysates, Lane 3: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F4 antigen affinity purified polyclonal antibody (Catalog # PB10057) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F4 at approximately 44 kDa, 60 kDa. The expected band size for E2F4 is at 44 kDa.
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