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ProductsBack/E2F4 Recombinant Antibody, AbBy Fluor-350 Conjugated

E2F4 Recombinant Antibody, AbBy Fluor-350 Conjugated

BSM-61764R-BF350
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetE2F4
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Overview

  • Supplier
    Bioss
  • Product Name
    E2F4 Recombinant Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), IF(), FCM(1ug/Test)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID1874
  • Target name
    E2F4
  • Target description
    E2F transcription factor 4
  • Target synonyms
    E2F-4, transcription factor E2F4, E2F transcription factor 4, p107/p130-binding, p107/p130-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16254
  • Protein Name
    Transcription factor E2F4
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of E2F4 using anti-E2F4 antibody (PB10057). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: U20S whole cell lysates, Lane 3: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F4 antigen affinity purified polyclonal antibody (Catalog # PB10057) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F4 at approximately 44 kDa, 60 kDa. The expected band size for E2F4 is at 44 kDa.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with E2F4 antibody (GTX134346) diluted at 1:1000 and competitor's antibody (sc-866) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
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