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anti-EAPP antibody

ARG43062
Arigo Biolaboratories
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEAPP
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-EAPP antibody
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55837
  • Target name
    EAPP
  • Target description
    E2F associated phosphoprotein
  • Target synonyms
    BM036, C14orf11, E2F-associated phosphoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a phosphoprotein that interacts with several members of the E2F family of proteins. The protein localizes to the nucleus, and is present throughout the cell cycle except during mitosis. It functions to modulate E2F-regulated transcription and stimulate proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EAPP using anti-EAPP antibody (A07290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human K562 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAPP antigen affinity purified polyclonal antibody (Catalog # A07290-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAPP at approximately 45KD. The expected band size for EAPP is at 45KD.
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