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Anti-EDA Antibody

144-02905
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEDA
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-EDA Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1896
  • Target name
    EDA
  • Target description
    ectodysplasin A
  • Target synonyms
    ECTD1; ectodysplasin-A; ED1; ED1-A1; ED1-A2; EDA1; EDA2; EDA-A1; EDA-A2; HED; HED1; ODT1; oligodontia 1; STHAGX1; TNLG7C; tumor necrosis factor ligand 7C; XHED; XLHED; X-linked anhidroitic ectodermal dysplasia protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92838
  • Protein Name
    Ectodysplasin-A
  • Scientific Description
    EDA Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EDA using anti-EDA antibody (PB9191). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDA antigen affinity purified polyclonal antibody (Catalog # PB9191) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDA at approximately 41 kDa. The expected band size for EDA is at 41 kDa.
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