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Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.
Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.
Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.

Anti-EDA Antibody

HPA037972
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetEDA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EDA Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1896
  • Target name
    EDA
  • Target description
    ectodysplasin A
  • Target synonyms
    ECTD1, ED1, ED1-A1, ED1-A2, EDA-A1, EDA-A2, EDA1, EDA2, HED, HED1, ODT1, STHAGX1, TNLG7C, XHED, XLHED, ectodysplasin-A, X-linked anhidroitic ectodermal dysplasia protein, oligodontia 1, tumor necrosis factor ligand 7C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92838
  • Protein Name
    Ectodysplasin-A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of EDA using anti-EDA antibody (PB9191). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDA antigen affinity purified polyclonal antibody (Catalog # PB9191) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDA at approximately 41 kDa. The expected band size for EDA is at 41 kDa.
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