Anti-EGFR [cet.Hum (Cetuximab - humanized; hC225)]

Absolute Antibody

Anti-EGFR [cet.Hum (Cetuximab - humanized; hC225)]

9

This antibody recognizes human EGFR. The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.

The binding characterization of this humanized scFv version of cetuximab to human EGFR was done using ELISA. This antibody can recognize human EGFR from lysates of A-431 and MCF-7 cells in western blot. The scFv version was able to recognize EGFR on over-expressing cancer cells (A-431) but failed to detect cancer cells with low levels of EGFR (MCF-7 cells) in a flow cytometric experiment. The binding affinity of this scFv version was reported to be 9 fold lower than that of the parental cetuximab, but still it was strong enough for the successful recognition of human EGFR expressing cells. This antibody can be used to block cancer cell surface receptors and inhibit their downstream signaling (PMID: 29185855).

Flow Cytometry, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

cet.Hum (Cetuximab - humanized; hC225)

EGFR

epidermal growth factor receptor

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

Epidermal growth factor receptor

Human

-20°C,2°C to 8°C

12352203