Anti-EGFRvIII [806], Rabbit IgG, kappa

Absolute Antibody

Anti-EGFRvIII [806], Rabbit IgG, kappa

9

This antibody is specific for the human delta2-7 epidermal growth factor receptor (de2-7 EGFR or EGFRvIII) which is a truncated version of the EGFR commonly expressed in glioma. This antibody also binds overexpressed wild-type (wt) EGFR but not native EGF

This antibody bound de2-7 EGFR (EGFRvIII) transfected U87MG glioma cells with high affinity but did not bind parental cells that express the wild type EGFR in a flow cytometric assay. This antibody can bind wild type EGFR containing the extracellular domain only in certain conditions like when the extracellular domain was immobilized on the surface of ELISA plates (PMID: 11920591). The antitumor activity of this antibody against human tumor xenografts grown in nude mice was studied. This antibody significantly inhibited the growth of U87 MG xenografts transfected with the de2-7 EGFR in a dose-dependent manner. It further also inhibited growth of new and established A431 xenografts, a cell line expressing >10^6 EGFRs/cell (PMID: 11454674). The efficacy of this antibody, on the growth of intracranial xenografted gliomas in nude mice was also studied. It was reported that treatment of U87 MG.DEGFR tumors in mice with mAb 806 caused decreases in both tumor growth and angiogenesis, as well as increased apoptosis. In vivo mAb 806 treatment resulted in reduced phosphorylation of the constitutively active DEGFR and caused down-regulated expression of the apoptotic protector, Bcl-XL (PMID: 11454673).

Flow Cytometry, ELISA

Research Use Only

Monoclonal

806

EGFR

epidermal growth factor receptor

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

Rabbit

IgG

Epidermal growth factor receptor

Human

-20°C,2°C to 8°C

12352203