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Immunohistochemical staining of human Breast shows strong membranous positivity in adipocytes.
Immunohistochemical staining of human Breast shows strong membranous positivity in adipocytes.
Immunohistochemical staining of human Breast shows strong membranous positivity in adipocytes.

Anti-EHD2 Antibody

HPA047394
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetEHD2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EHD2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30846
  • Target name
    EHD2
  • Target description
    EH domain containing 2
  • Target synonyms
    EH domain-containing protein 2; PAST homolog 2; PAST2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZN4
  • Protein Name
    EH domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EHD2 using anti-EHD2 antibody (A04265-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD2 antigen affinity purified polyclonal antibody (Catalog # A04265-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD2 at approximately 61 kDa. The expected band size for EHD2 is at 51 kDa.
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