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Western Blot Positive WB detected in: Hela whole cell lysate, Mouse liver tissue, Mouse kidney tissue All lanes: EHD2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62, 47 kDa Observed band size: 62, 47 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse liver tissue, Mouse kidney tissue All lanes: EHD2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62, 47 kDa Observed band size: 62, 47 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse liver tissue, Mouse kidney tissue All lanes: EHD2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62, 47 kDa Observed band size: 62, 47 kDa

EHD2 Antibody

CSB-PA873710LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEHD2
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Overview

  • Supplier
    Cusabio
  • Product Name
    EHD2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30846
  • Target name
    EHD2
  • Target description
    EH domain containing 2
  • Target synonyms
    EH domain-containing protein 2; PAST homolog 2; PAST2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZN4
  • Protein Name
    EH domain-containing protein 2
  • Scientific Description
    ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed:17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EHD2 using anti-EHD2 antibody (A04265-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD2 antigen affinity purified polyclonal antibody (Catalog # A04265-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD2 at approximately 61 kDa. The expected band size for EHD2 is at 51 kDa.
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