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Immunohistochemical staining of human urinary bladder shows moderate membranous and cytoplasmic positivity in urothelial cells, as well as in endothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate membranous and cytoplasmic positivity in urothelial cells, as well as in endothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate membranous and cytoplasmic positivity in urothelial cells, as well as in endothelial cells.

Anti-EHD4 Antibody

HPA052729
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEHD4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EHD4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30844
  • Target name
    EHD4
  • Target description
    EH domain containing 4
  • Target synonyms
    PAST4, EH domain-containing protein 4, PAST homolog 4, hepatocellular carcinoma-associated protein 10/11, hepatocellular carcinoma-associated protein HCA11, ortholog of rat pincher
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H223
  • Protein Name
    EH domain-containing protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EHD4 using anti-EHD4 antibody (A07430-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey heart tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD4 antigen affinity purified polyclonal antibody (Catalog # A07430-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD4 at approximately 70 kDa. The expected band size for EHD4 is at 61 kDa.
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