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EHD4 Antibody

LS-C748799
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetEHD4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EHD4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 61kDa, while the observed MW by Western blot was 72kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 61kDa, while the observed MW by Western blot was 72kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID30844
  • Target name
    EHD4
  • Target description
    EH domain containing 4
  • Target synonyms
    PAST4, EH domain-containing protein 4, PAST homolog 4, hepatocellular carcinoma-associated protein 10/11, hepatocellular carcinoma-associated protein HCA11, ortholog of rat pincher
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EHD4 using anti-EHD4 antibody (A07430-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey heart tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD4 antigen affinity purified polyclonal antibody (Catalog # A07430-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD4 at approximately 70 kDa. The expected band size for EHD4 is at 61 kDa.
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